Tag Archives: pertussis

Oral and Nasopharyngeal Specimen Collection


This is a brief list of oral and nasopharyngeal specimen collection procedures.

MRSA

  • Location: Anterior nares
  • Swab: Routine cotton culture swab

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Influenza

  • Location: Posterior nasopharynx
  • Swab: Routine cotton culture swab
  • Wear a surgical mask, protective eye wear and gloves while collecting the specimen
  • Place the patients head on the bed pillow or against a wall so that they do not move backwards
  • The swab will go straight backwards like placing an NG tube, it should go the distance between the nostril and the ear into the throat.
  • The swab is rotated for a bout 5–10 seconds to get enough epithelial cells on it
  • Remove the swab and place it in the transport media and sent it to lab

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Pertussis

The PCR assay reuires a special swab, made of aluminum with a nylon tip. Traditional cotton tip swabs interfere with the test

  • Location: Posterior nasopharynx
  • A special transport media is needed- call the lab for it
  • Wear a surgical mask, protective eye wear and gloves while collecting the specimen
  • Place the patients head on the bed pillow or against a wall so that they do not move backwards
  • The swab will go straight backwards like placing an NG tube, it should go the distance between the nostril and the ear into the throat. This is different from a nasal swab for MRSA or influenza
  • The swab is rotated for a bout 5–10 seconds to get enough epithelial cells on it
  • Remove the swab and place it in the transport media and sent it to lab

Pertussis-2

Here is a video on specimen collection

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Mumps

  • Location: Specimen is collected from the parotid duct secretions preferrably within 9 days of symptoms.
  • Swab: Routine cotton swab. Specimen is sent in a viral transport media.
  • Steps
    • Massage the parotid gland for 30 seconds. Note that it is just in front of the ear lobe. CDR742039
    • Specimen should be collected from the parotid duct opening just next to the second molar.
      mumps-cavity

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Increase in pertussis cases in Harvard, IL

McHenry County Department of Health (MCDH) has noticed an increase on cases of pertussis in Harvard IL.  Please be aware of this vaccine preventable disease when evaluating any child or adult with acute cough like illness characterized by dry hacking cough which may be worse at night associated with paroxysms, a whoop or post tussle gagging or vomiting. Especially if symptoms are lasting greater than 2 weeks.

Please remember to start treatment even before laboratory confirmation

Preferred treatment included – azithromycin, erythromycin or clarithromycin.

Please note the correct way to collect specimen as shown in this video

Pertussis cases should be reported to the county health department using the attached Pertussis_Reporting_Form.

Victories in Infectious Diseases – Video of lecture

This lecture was presented at the July 2013 Centegra summer symposium. Continue reading

Isolation guidelines

A page for Isolation guidelines is now available under Departments >> Infection Control >> Isolation guidelines.

Immunization – myths and misconceptions (2012)

Myths of immunization

This lecture was presented at the recent fall nursing symposium.

References and links

  1. Inflammation described by Celsus
  2. What is an antigen?
  3. Types of immunity
  4. Plague in the middle ages
  5. How India has defeated Polio, a BBC report
  6. Edward Jenner – the man who saved more lives than any other man
  7. Herd immunity
  8. Algorithm for immunizing persons with egg allergies
  9. Influenza vaccination in individuals with egg allergies
  10. Ileal-lymphoid nodular hyperplasia, non-specific colitis and pervasive developmental disorder in children, Lancet 1998- the original article by AJ Wakefield that started the controversy over vaccination in the late 1990s
  11. Wikipedia article on Andrew Wakefield
  12. My comments on Wakefield findings dated January 2011
  13. Article in The Telegraph reporting on the outbreak of Measles in Liverpool
  14. Risk of seizures after whole cell pertussis or Measles, Mumps, and Rubella vaccine
  15. Gates foundation and vaccination

Pertussis lecture 2012

Pertussis lecture

2012 update on Pertussis (Whooping cough)


This is the recording of a lecture that I gave to the hospital staff on Pertussis.

References and links

  1. CDC page on pertussis
  2. Collection of nasopharyngeal swab for pertussis
  3. Risk of seizures after whole cell pertussis or Measles, Mumps, and Rubella vaccine
  4. Ileal-lymphoid nodular hyperplasia, non-specific colitis and pervasive developmental disorder in children, Lancet 1998- the original article by AJ Wakefield that started the controversy over vaccination in the late 1990s
  5. Wikipedia article on Andrew Wakefield
  6. Article in The Telegraph reporting on the outbreak of Measles in Liverpool

Pertussis specimen collection

Collecting a pertussis specimen

We had a bit of confusion of pertussis collection this weekend, so I put together a brief overview on pertussis with focus on nasopharyngeal specimen collection for pertussis.
Continue reading